1 Registry Star
Generator
SG
Part:BBa_J45320:Experience
Designed by: André Green II Group: iGEM06_MIT (2006-10-20)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45320
Production of the wintergreen odor precursor salicylic acid.
User Reviews
UNIQ256970025e762d43-partinfo-00000000-QINU
|
••• |
BBa_J45320, when used in the wintergreen odor biosynthetic system BBa_J45700, appears to produce some salicylate from the cellular metabolite chorismate. |
UNIQ256970025e762d43-partinfo-00000004-QINU
Characterization
Gas chromatography analysis of wintergreen odor biosynthetic system
The wintergreen odor biosynthetic system (BBa_J45700) includes the salicylate generator BBa_J45320.
Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.